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KMID : 0380020170320010016
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 1 p.16 ~ p.21
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Production of Human Interferon β by Recombinant E. coli Using the Codon Optimized Gene
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Kim Jong-Seok
Jang Seung-Won
Park Jae-Bum
Kwon Deok-Ho
Chang Young-Jun
Jung Hyung-Moo
Han Sang-In
Hong Eock-Kee
Ha Suk-Jin
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Abstract
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The multiple sclerosis caused by multiple inflammatory disease or immune system disorder, is usually treated by interferon β through adjusting the abnormal immune reactions. For high production of human interferon β using recombinant E. coli, codon optimized and wild type genes were synthesized. When pET-15b or pET-21a vector was used as an expression vector with each gene, there was no target protein expression. When pQE30 vector was used as an expression vector, human interferon β was expressed by recombinant E. coli XL1-blue and E. coli JM109. Using the codon optimized gene, the expression of human interferon β was slightly increased as compared to that from wild type gene. However, most of expressed human interferon β was insoluble form.
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KEYWORD
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Human interferon β, Recombinant E. coli, Codon optimization
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